qPCR Human Reference cDNA, Oligo(dT)-primed
qPCR Human Reference cDNA, Oligo(dT)-primed
Brand: Takara Bio.
In stock
SKU
qPCR Human Reference cDNA, Oligo(dT)-primed
Reference cDNA for real-time qPCR
cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.
qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.
The sample was oligo(dT) primed.
Overview
- Broad gene coverage
- Virtually free of genomic DNA
- Made from human tissues, not cultured cell lines
- A high-performance standard for quantitative PCR
Applications
- Real-time PCR/qPCR
- Gene expression analysis
- One-step RT-PCR
- Northern analysis
- Microarray analysis
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting Ct values versus template quantity
Reference
Suzuki, T., Higgins, P. J. & Crawford, D. R. Control selection for RNA quantitation. Biotechniques 29, 332–7 (2000).
Write Your Own Review
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting Ct values versus template quantity
