pZsYellow Vector
pZsYellow Vector
pZsYellow is a prokaryotic expression vector, which encodes Zoanthus sp. yellow fluorescent protein (ZsYellow). The ZsYellow coding sequence carried in pZsYellow retains the wild-type codon usage. In this vector, the ZsYellow gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the ZsYellow gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of ZsYellow cDNA.
Overview
- Bright yellow fluorescent protein
- ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively
Applications
- Fusions
- Protein localization
- General reporter for mammalian cells
- Monitoring transfection efficiencies
Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Four-color visualization of Living Colors Fluorescent Proteins
Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.