pZsGreen1-N1 Vector
pZsGreen1-N1 Vector
pZsGreen1-N1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. green fluorescent protein, ZsGreen1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsGreen1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the ZsGreen1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express ZsGreen1 in mammalian cells.
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Fluorescent whole-body image of a mouse with bilateral solid tumors derived from estrogen receptor-positive MCF7 human breast cancer cells
Fluorescent whole-body image of a mouse with bilateral solid tumors derived from estrogen receptor-positive MCF7 human breast cancer cells. Note: the presence of ZsGreen-positive cells in tumors, lymphatic vessels (LV), and inguinal and axillary lymph nodes (LN).
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.