pUC18 and pUC19 Vectors
pUC18 and pUC19 Vectors
Brand: Takara Bio.
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pUC18 and pUC19 Vectors
pUC18 and pUC19 vectors
The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a multiple cloning site at the lacZ' region that enables recombinant plamids to be verified via blue/white colony screening using agar plates containing IPTG and X-Gal. Expression of target DNA is enabled by the presence of a lac promoter in the cloning vectors. The pUC18 and pUC19 plasmids are suitable for dideoxy DNA sequencing using M13 primers. To sequence pUC18 and pUC19 plasmids containing large DNA insertions, utilize the Deletion Kit for Kilo-Sequencing (Cat. # 6030).
Applications
- Cloning and expression of target genes
- DNA sequencing using M13 primers
- Large DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing
Purity
- >70% double-stranded, covalently closed circular form I (RF I) DNA.
- Multiple cloning site sequence verified by dideoxy DNA sequencing.
- Restriction site verified by restriction enzyme cleavage.
Storage
–20°C
Concentration
250–1,000 µg/ml
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Size
2,686 bp
GenBank
pUC18/19 Type Vector Map
pUC18/19 Type Vector Map.
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Purity
- >70% double-stranded, covalently closed circular form I (RF I) DNA.
- Multiple cloning site sequence verified by dideoxy DNA sequencing.
- Restriction site verified by restriction enzyme cleavage.
Storage
–20°C
Concentration
250–1,000 µg/ml
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Size
2,686 bp
GenBank
pUC18/19 Type Vector Map
pUC18/19 Type Vector Map.
