ptdTomato-N1 Vector
ptdTomato-N1 Vector
Brand: Takara Bio.
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ptdTomato-N1 Vector
ptdTomato-N1 is a mammalian expression vector containing a tandem sequence of the dimeric red fluorescent protein, Tomato. An MCS at the 5' end of the coding sequence makes it possible to insert a gene of interest in order to express it as an N-terminal fusion with tdTomato. The unmodified vector can be used to express tdTomato in mammalian cells.
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
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Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
