pT7-IRES Vectors
pT7-IRES Vectors
Brand: Takara Bio.
In stock
SKU
pT7-IRES Vectors
pT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES.
pT7-IRES vectors
Each vector (Cat. # 3290, 3291, 3292) contains 20 µg DNA at a concentration of 0.5 µg/µl (40 µl total volume).
| Human Cell-Free Protein Expression Maxi System | |
|---|---|
| 1) Human Cell-Free Protein Expression System (Cat. # 3281) | 2 kits |
| 2) Supplement Buffer (Human Cell-Free Protein Expression) | 15 ml |
| 3) Dialysis Columns & Accessories | 25 sets |
Notes:
- Items (2) and (3) are not available for sale separately.
- Item (3) is manufactured by G-Biosciences and includes the following components:
- Tube-O-DIALYZER™, Micro 15K MWCO (5 tubes) Note: Each tube is filled with a preservative solution. The shipping package also contains a small amount of preservative solution to maintain moisture.
- Micro Dialysis Cup (5 cups)
- Float for Micro, for Dialysis Cup (5 floats)
pT7-IRES vector information
Vectors in the pT7-IRES series include a T7 promoter and EMCV IRES sequence to facilitate transcription and translation in the Human Cell-Free Protein Expression System as well as a Multiple Cloning Site (MCS), convenient tags (N- or C-terminal His or N-terminal Myc tag sequences), a Factor Xa protease cleavage site for tag removal, polyA signal and T7 terminator.
- pT7-IRES His-N DNA (Cat. # 3290) encodes an N-terminal His tag
- pT7-IRES His-C DNA (Cat. # 3291) encodes a C-terminal His tag
- pT7-IRES Myc-N DNA (Cat. # 3292) encodes an N-terminal c-Myc tag
Write Your Own Review
