ProteoTuner Tag Kit
ProteoTuner Tag Kit
Brand: Takara Bio.
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ProteoTuner Tag Kit
The ProteoTuner Tag Kit provides you with a ProteoTuner tag sequence so you can quickly and easily convert any Living Colors fluorescent protein-C vector into a ProteoTuner vector. The Kit contains a ProteoTuner destabilization domain (DD) source vector plus a primer mix that lets you PCR-amplify the DD tag sequence. You can then use an In-Fusion Cloning system to insert the DD tag sequence upstream of the fluorescent protein coding sequence in any of our fluorescent protein-C vectors linearized at the AgeI site. The resulting vector will be able to express a protein of interest that is N-terminally-tagged with a fluorescent protein and subject to ligand-dependent degradation.
Overview
- Rapid kinetics: protein level changes in minutes allows accurate functional analysis
- Precise tuning: precise control of protein level by controlling the dose of Shield1
- Reversible control: "protein on" to "protein off" for convincing gene-function studies
- What you get: each kit is supplied with a plasmid vector and an aliquot of Shield1.
- NOTE: Most of the proteins that we tested showed a better destabilization profile when the DD tag was fused to the N-terminus of the protein of interest (Systems N). Specific DD tag mutants for C-terminal tagging are available as well (System C); however they have a slightly reduced destabilization activity in the absence of the Shield1 ligand.
Applications
- Protein function in pathways
- Functional analysis of subunits of a protein complex
- Functional analysis of essential proteins
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