ProteoGuard EDTA-Free Protease Inhibitor Cocktail
ProteoGuard EDTA-Free Protease Inhibitor Cocktail
Brand: Takara Bio.
In stock
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ProteoGuard EDTA-Free Protease Inhibitor Cocktail
Complete protease inhibitor cocktail
ProteoGuard EDTA-Free Protease Inhibitor Cocktail is a complete, optimized mixture of protease inhibitors specially designed to protect proteins from being digested by endogenous proteases that can be released during protein extraction from cell lysates.
- Contains an optimized mixture of five different inhibitors
- Supplied as a convenient 100X stock in DMSO
Flexible packaging
The ProteoGuard EDTA-Free Protease Inhibitor Cocktail 100X stock solution is easy to use and is prepacked for single-use applications. It is not supplied in a tablet format, so there is no need to wait for the tablet to dissolve before use. Just dispense the required amount of protease inhibitors (10 µl per 1 ml) into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast, or bacteria. ProteoGuard EDTA-Free Protease Inhibitor Cocktail is compatible with a variety of cell lysis reagents.
Better performance than Competitor P
A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).
Overview
- Complete EDTA-free protease inhibitor cocktail
- Suppresses proteolysis in cell lysates
- Outstanding performance when used with xTractor Buffer or other lysis buffers
- Flexible packaging—single-use tubes
Applications
- Protein purification
- Suppression of proteolysis in detergent-based cell-lysis reagents
- Inhibition of protease activity from lysates produced by other lysis methods
Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract
Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).
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Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract
Better Performance than Competitor P—A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).
