PrimeSTAR Max DNA Polymerase
PrimeSTAR Max DNA Polymerase
Brand: Takara Bio.
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PrimeSTAR Max DNA Polymerase
Choose for speed, convenience and highest fidelity. 2X hot-start PCR master mix ideal for HTP applications.
PrimeSTAR Max DNA Polymerase
A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.
Overview
- Highest fidelity of any commercially available DNA polymerase
- Fastest extension speed means less time required for PCR cycles
- Convenient 2X premix includes buffer and dNTPs, allowing reaction assembly in less time
- Antibody-mediated hot-start formulation
- Proven performance as reported in peer-reviewed literature
Applications
- Fast PCR for high-throughput studies
- Cloning and expression studies
- Structure/function studies
- Evolutionary studies (e.g., SNP analyses)
PCR product termini
- PrimeSTAR Max DNA Polymerase produces products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.
Recommended maximum amplicon length
- Up to 6 kb (human genomic DNA template or human cDNA template)
- Up to 10 kb (E. coli genomic DNA template)
- Up to 15 kb (lambda DNA template)
The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data
The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data. Eight arbitrarily selected GC-rich regions were amplified with PrimeSTAR Max DNA Polymerase or other DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. PCR products (~500 bp each) were each cloned into a suitable plasmid. Multiple clones were selected per respective amplification product and were subjected to sequence analysis. Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNA Polymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.
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The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data
The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data. Eight arbitrarily selected GC-rich regions were amplified with PrimeSTAR Max DNA Polymerase or other DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. PCR products (~500 bp each) were each cloned into a suitable plasmid. Multiple clones were selected per respective amplification product and were subjected to sequence analysis. Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNA Polymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.
