Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.

Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.

Overview

• Manufactured and labeled in accordance with requirements for general purpose reagents
• Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
• Highest processivity of all commercially-available high-fidelity DNA polymerases
• Antibody-mediated hot-start formulation promotes successful, robust, and high-fidelity PCR regardless of conditions
• Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
• Easy amplification of GC- or AT-rich templates and targets containing repeats
• High tolerance for GC-rich templates, excess nucleic acids, and amplification of long products
• Buffer and dNTPs are conveniently provided in the kit

Applications

• Amplification prior to NGS
• PCR with GC-rich, AT-rich, or repetitive sequences
• Cloning
• Long-range PCR
• Sequencing
• Rare-template amplification
• PCR with limiting sample amounts