PrimeSTAR GXL Premix

This optimized, 2X master mix is composed of PrimeSTAR GXL DNA Polymerase (a high-fidelity DNA polymerase), reaction buffer, and dNTP mixture. PrimeSTAR GXL Premix is a robust enzyme that can be used in difficult reactions, including amplification of GC-rich regions (without requiring special reaction conditions), in the presence of excess template, and with long targets up to 30 kb in length.

Overview

• Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
• Antibody-mediated hot-start formulation
• High tolerance for GC-rich templates as well as the presence of excess nucleic acid templates

Applications

• Realiable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
• HLA typing
• Unbiased library amplification for NGS

Termini of PCR products

PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.