PrimeScript RT Reagent Kit with gDNA Eraser
PrimeScript RT Reagent Kit with gDNA Eraser
Brand: Takara Bio.
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PrimeScript RT Reagent Kit with gDNA Eraser
PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time): Eliminate genomic DNA contamination
PrimeScript RT reagent Kit with gDNA Eraser is a reverse-transcription kit for real-time RT-PCR (RT qPCR) that includes a genomic DNA elimination reaction. cDNA synthesis from RNA can be achieved without sample loss in a rapid reaction that is complete in less than 20 minutes. Genomic DNA is eliminated by treatment for 2 minutes at 42℃ with gDNA Eraser, which has potent DNA degradation activity. Then a reverse-transcription reaction reagent is added that includes a component which completely inhibits DNA degradation activity, and the reverse-transcription reaction proceeds for 15 minutes. The cDNA obtained using this product can be used with either an intercalating green dye qPCR assay or probe qPCR assay. Please use in combination with quantitative PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A) and Probe qPCR Mix (Cat. #RR391A).
Overview
- Genomic DNA contamination elimination in only 2 minutes
- Fast cDNA synthesis—reverse transcription in just 15 minutes
- Suitable for use with routine and challenging RNA templates alike
- Full-length cDNA synthesis up to 12 kb
Applications
- Elimination of genomic DNA contamination
- cDNA synthesis for real-time RT-PCR (qPCR)
- Two-step real-time RT-PCR
Product citations
Fukui, K. et al. Selective mimics of strigolactone actions and their potential use for controlling damage caused by root parasitic weeds. Mol. Plant 6, 88–99 (2013).
Nakajima, A. et al. Lon protease of Azorhizobium caulinodans ORS571 is required for suppression of reb gene expression. Appl. Environ. Microbiol. 78, 6251–61 (2012).
Nemoto, T., Mano, A. & Shibasaki, T. Increased expression of miR-325-3p by urocortin 2 and its involvement in stress-induced suppression of LH secretion in rat pituitary. Am. J. Physiol. - Endocrinol. Metab. 302, (2012).
Qin, P. et al. ABCG15 encodes an ABC transporter protein, and is essential for post-meiotic anther and pollen exine development in rice. Plant Cell Physiol. 54, 138–154 (2013).
Sasaki, N. et al. The splice variant Ntr encoded by the tobacco resistance gene N has a role for negative regulation of antiviral defense responses. doi:10.1016/j.pmpp.2013.08.002
Sato, Y. et al. Anks4b, a novel target of HNF4α protein, interacts with GRP78 protein and regulates endoplasmic reticulum stress-induced apoptosis in pancreatic β-cells. J. Biol. Chem. 287, 23236–45 (2012).
Wang, Q. et al. Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster. J. Biol. Chem. 287, 6159–68 (2012).
Xiang, J.-J., Zhang, G.-H., Qian, Q. & Xue, H.-W. Semi-rolled leaf1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells. Plant Physiol. 159, 1488–500 (2012).
Yang, Y. et al. Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system. Appl. Environ. Microbiol. 78, 5845–54 (2012).
Zhou, M.-L. et al. Genome-wide identification of genes involved in raffinose metabolism in Maize. Glycobiology 22, 1775–1785 (2012).
Zhou, Z. et al. Follicular development and expression of nuclear respiratory factor-1 and peroxisome proliferator-activated receptor coactivator-1 alpha in ovaries of fetal and neonatal doelings. J. Anim. Sci. 90, 3752–3761 (2012).
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