PrimeScript Two Step RT-PCR Kit
PrimeScript Two Step RT-PCR Kit
Brand: Takara Bio.
In stock
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PrimeScript Two Step RT-PCR Kit
This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq HS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR.
Overview
- RT-PCR kit includes all reagents needed for two-step endpoint RT-PCR (e.g., where products are analyzed using gel electrophoresis)
- Robust reverse transcription and amplification even from challenging RNA templates
- Complete elongation of complex RNA templates
- High-yield transcription of full-length cDNA up to 12 kb in length
- Minimal false priming and robust amplification due to the combination of PrimeScript RT with Takara Ex Taq HS
Applications
- Two-step RT-PCR
RT-PCR using various amounts of RNA template
RT-PCR using various amounts of RNA template. To illustrate the high sensitivity of the PrimeScript™ RT-PCR Kit in detecting mRNAs in very low quantities of total RNA template, GAPDH mRNA was used as a target in various amounts of HL60 cell total RNA (0.1 pg to 1 ng). The RNA was reverse transcribed using the oligo dT primer. The kit protocol was followed for RT and PCR.
Amplifying various lengths of target gene mRNAs by RT-PCR
Amplifying various lengths of target gene mRNAs by RT-PCR. Using human heart total RNA or HL60 cell total RNA as a template, a variety of sequence lengths from two target gene mRNAs (dystrophin and transferrin receptor) were reverse transcribed and amplified by PCR using the reagents in the PrimeScript™ RT-PCR Kit.
Principle of PrimeScript RT-PCR Kit
Principle of PrimeScript RT-PCR Kit.
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RT-PCR using various amounts of RNA template
RT-PCR using various amounts of RNA template. To illustrate the high sensitivity of the PrimeScript™ RT-PCR Kit in detecting mRNAs in very low quantities of total RNA template, GAPDH mRNA was used as a target in various amounts of HL60 cell total RNA (0.1 pg to 1 ng). The RNA was reverse transcribed using the oligo dT primer. The kit protocol was followed for RT and PCR.
Amplifying various lengths of target gene mRNAs by RT-PCR
Amplifying various lengths of target gene mRNAs by RT-PCR. Using human heart total RNA or HL60 cell total RNA as a template, a variety of sequence lengths from two target gene mRNAs (dystrophin and transferrin receptor) were reverse transcribed and amplified by PCR using the reagents in the PrimeScript™ RT-PCR Kit.
Principle of PrimeScript RT-PCR Kit
Principle of PrimeScript RT-PCR Kit.
