PrimeScript One Step RT-PCR Kit
PrimeScript One Step RT-PCR Kit
Brand: Takara Bio.
In stock
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PrimeScript One Step RT-PCR Kit
This one-step RT-PCR kit allows efficient, specific cDNA synthesis and amplification in a single tube for endpoint analysis (e.g., gel electrophoresis). The kit features a streamlined workflow that does not require addition of reagents between the cDNA synthesis and amplification steps. The PrimeScript One Step RT-PCR Kit includes PrimeScript RT, an RNAse H-minus MMLV reverse transcriptase that allows robust reverse transcription of almost any RNA template, including GC-rich or highly structured RNA. To enable cDNA amplification, this one-step RT-PCR kit also includes Takara Ex Taq hot-start (HS) DNA polymerase, a thermostable polymerase that allows robust, specific amplification and is formulated for hot-start PCR. Thanks to the PrimeScript One Step Enzyme Mix, sample handling time and the risk of contamination are minimized. The PrimeScript One Step RT-PCR Kit (Dye Plus) also includes electrophoresis loading dyes (blue and yellow) in the premix to further reduce sample handling steps and allow visual confirmation of gel loading.
Overview
- Efficient, fast RT-PCR in a single tube
- Works efficiently with GC-rich and highly structured RNA templates
- Takara Ex Taq HS allows specific amplification with minimal background
- Quick and easy reaction preparation
- Choose from one-step RT-PCR kits with or without gel loading buffer included in the reaction
Applications
- One-step RT-PCR
- Gene expression analysis by endpoint RT-PCR
Components
- RR055A (50 reactions; 50 µl per reaction)
| PrimeScript One Step Enzyme Mix | 100 µl |
| 2X One Step Buffer | 2 × 625 µl |
| Control F-1 Primer* (20 µM) | 20 µl |
| Control R-1 Primer** (20 µM) | 20 µl |
| Positive Control RNA (2 x 105 copies/µl) | 20 µl |
| RNase Free dH2O | 2 × 625 µl |
* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA
- RR055B (200 reactions; 50 µl per reaction)
This kit is comprised of 4 x RR055A.
- RR057A (50 reactions; 50 µl per reaction)
| PrimeScript One Step Enzyme Mix | 100 µl |
| 2X One Step Buffer (Dye Plus) | 2 × 625 µl |
| Control F-1 Primer* (20 µM) | 20 µl |
| Control R-1 Primer** (20 µM) | 20 µl |
| Positive Control RNA (2 x 105 copies/µl) | 20 µl |
| RNase Free dH2O | 2 × 625 µl |
* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA
- RR057B (200 reactions; 50 µl per reaction)
This kit is comprised of 4 x RR057A.
Ishii, A. et al. Molecular surveillance and phylogenetic analysis of Old World arenaviruses in Zambia. J. Gen. Virol. 93, 2247-2251 (2012).
Kato, Y., Sawata, S. Y. & Inoue, A. A lentiviral vector encoding two fluorescent proteins enables imaging of adenoviral infection via adenovirus-encoded miRNAs in single living cells. J. Biochem. 147, 63-71 (2010).
Lu, P. et al. Silibinin attenuates amyloid beta(25-35) peptide-induced memory impairments: implication of inducible nitric-oxide synthase and tumor necrosis factor-alpha in mice. J. Pharmacol. Exp. Ther. 331, 319-326 (2009).
Morio, F., Pagniez, F., Lacroix, C., Miegeville, M. & Le pape, P. Amino acid substitutions in the Candida albicans sterol Δ5,6-desaturase (Erg3p) confer azole resistance: Characterization of two novel mutants with impaired virulence. J. Antimicrob. Chemother. 67, 2131-2138 (2012).
Yamayoshi, S., Ohka, S., Fujii, K. & Koike, S. Functional comparison of SCARB2 and PSGL1 as receptors for enterovirus 71. J. Virol. 87, 3335-47 (2013).
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