PrimeScript High Fidelity RT-PCR Kit
PrimeScript High Fidelity RT-PCR Kit
Brand: Takara Bio.
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PrimeScript High Fidelity RT-PCR Kit
The PrimeScript High Fidelity RT-PCR Kit is designed to synthesize and amplify cDNA from total RNA or mRNA with high fidelity. This kit includes all reagents necessary for reverse transcription of RNA to cDNA followed by PCR amplification of cDNA, including PrimeScript reverse transcriptase and PrimeSTAR Max DNA Polymerase.
Overview
- Exceptionally low error rates during reverse transcription and PCR amplification
- Excellent extension capacity even with highly structured template RNAs containing higher-order structures
- Broadly tolerates a wide range of input RNA template concentrations
Applications
- RT-PCR gene expression studies
- Full-length high-fidelity cDNA synthesis and analysis by endpoint PCR (gel electrophoresis)
- Two-step RT-PCR
- cDNA library construction
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