cDNA cloning is used for structural analysis of genes and RNAs, and expression of target proteins. Generating a cDNA library involves synthesizing double-stranded cDNA that is complementary to the target mRNA, inserting the cDNA into a vector of bacterial or viral origin, and introducing the vector into bacterial or eukaryotic cells. The cloned cDNA can be then used for analysis, and in vitro transcription and translation. The PrimeScript Double Strand cDNA Synthesis Kit synthesizes double-stranded cDNA from polyA+ RNA of animal or plant origin using the Gubler-Hoffman method.
Overview
- Includes high efficiency PrimeScript Reverse Transcriptase to synthesize first-strand cDNA from purified poly A+ RNA
- The resulting double-stranded cDNA can be cloned into an appropriate vector to generate a cDNA library
- This kit contains positive control RNA, which can be cloned into a selectable plasmid to confirm success
Applications
- Synthesis of double-stranded cDNA using the Gubler-Hoffman method (Gubler and Hoffman 1983)
- Construction of cDNA libraries
