PrimerArray Series for Biological Pathways
PrimerArray Series for Biological Pathways
Brand: Takara Bio.
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PrimerArray Series for Biological Pathways
PrimerArray series for biological pathways
Takara Bio's PrimerArray Series offers primer sets for real-time RT-PCR (qPCR) that can be used for gene expression analysis of biological pathways. Each PrimerArray Series primer set contains 96 total primers that allow for the simultaneous analysis of 88 biological pathway-related genes and 8 housekeeping genes. The performance of each primer set for real-time RT-PCR (qPCR) has been validated.
The PrimerArray Analysis Tool* (download) is capable of analyzing data obtained using PrimerArray Series products. This analysis tool is linked to the KEGG (Kyoto Encyclopedia of Genes and Genomes) database, allowing results to be visualized on a pathway map. Primer set information and a manual on how to use the tool can be downloaded by opening the product table and clicking on the Resources tab for your product of interest.
Overview
- Affordability: Each primer set for real-time RT-PCR (qPCR) supplies sufficient primer for up to 12 qPCR experiments (using a 25 µl qPCR reaction volume)
- Minimizes pipetting steps and reduces error
- Enables high-throughput gene expression analysis of biological pathways
- Optimized for superior performance with TB Green Ex Taq qPCR kits
- PrimerArray Analysis Tool makes data analysis easy and allows data visualization on pathway maps
- Compatible with all standard real-time PCR instruments
Applications
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Gene expression analysis of biological pathways including cytokine-cytokine receptor interactions, cell cycling, cell adhesion, Jak-STAT signaling, natural killer cell mediated cytotoxicity, axon guidance, focal adhesion, T cell receptor signaling, TGF-beta signaling, Wnt signaling.
Procedural overview for gene expression analysis by real-time RT-PCR
The overall workflow for the PrimerArray Series primer sets is straightforward:
- RNA extraction (30 min): NucleoSpin RNA (Cat. # 740955.50, not available in all geographic locations) is recommended.
- Reverse transcription (20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A) is recommended.
- Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat.# RR820A); dispense into a 96-well plate.
- Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction
- Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems
- Data analysis (30 minutes): Use the PrimerArray Analysis Tool
PrimerArray Analysis Tool
The PrimerArray Analysis Tool facilitates data analysis, and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
Available PrimerArray Primer Sets
Human
- PH001 - Cytokine-cytokine receptor interaction
- PH002 - Cell cycle
- PH003 - Cell adhesion molecules (CAMs)
- PH004 - Jak-STAT signaling pathway
- PH005 - Natural killer cell mediated cytotoxicity
- PH006 - Axon guidance
- PH007 - Focal adhesion
- PH009 - TGF-beta signaling pathway
- PH010 - Wnt signaling pathway
Mouse
- PN001 - Cytokine-cytokine receptor interaction
- PN002 - Cell cycle
- PN003 - Cell adhesion molecules
- PN004 - Jak-STAT signaling pathway
- PN005 - Natural killer cell mediated cytotoxicity
- PN006 - Axon guidance
- PN007 - Focal adhesion
- PN008 - T-cell receptor signaling pathway
- PN009 - TGF-beta signaling pathway
- PN010 - Wnt signaling pathway
Product citations
Hirano, S., Fujitani, Y., Furuyama, A. & Kanno, S. Uptake and cytotoxic effects of multi-walled carbon nanotubes in human bronchial epithelial cells. Toxicol. Appl. Pharmacol. 249, 8–15 (2010).
Yoshioka, R. et al. Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. Invest. Ophthalmol. Vis. Sci. 51, 5630–9 (2010).
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