PrimerArray Embryonic Stem Cell Primers
PrimerArray Embryonic Stem Cell Primers
Brand: Takara Bio.
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PrimerArray Embryonic Stem Cell Primers
Gene expression analysis to screen for pluripotency of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is streamlined and efficient with PrimerArray embryonic stem cell arrays. These arrays include primers for key markers of mouse or human ES cell pluripotency as determined by the International Stem Cell Initiative International (ISCI). When performing gene expression analysis by real-time RT-PCR, these ready-to-use primer sets save time, minimize pipetting, and reduce error.
Each PrimerArray embryonic stem cell array contains 96 primer pairs representing 88 genes associated with embryonic stem cell or iPS cell pluripotency and eight housekeeping genes. The PrimerArray Analysis Tool for Embryonic Stem Cells (available for download here) and/or Multiplate RQ (for use with the Thermal Cycler Dice Real Time Systems, not available in all geographic locations) are useful for analyzing data obtained using these products.
Overview
- Easily investigate the pluripotency of ES/iPS cells by real-time PCR: measure key markers of human or mouse embryonic stem cells as determined by the International Stem Cell Initiative International (ISCI)
- Affordable: each PrimerArray set supplies sufficient qPCR primers for up to 12 real-time PCR runs (25-µl reaction volume)
- Reduces error by minimizing pipetting
- Enables high-throughput analysis of stem cell pluripotency
- Optimized for superior performance with TB Green Ex Taq qPCR kits
- Straightforward data analysis with the PrimerArray analysis tool
- Compatible with all standard real-time PCR systems
Applications
- Pluripotency screens on embryonic stem cell and iPS cell samples
- Screening for expression of stem cell markers in response to conditions or agents affecting pluripotency
Procedural overview for stem cell gene expression analysis by real-time RT-PCR
The overall workflow for the PrimerArray sets is straightforward:
- RNA extraction (30 min): we recommend NucleoSpin RNA II kits (Cat. # 740955.50) or RNAiso (Cat. # 9108, not available in all geographic locations)
- Reverse transcription (20 min): we recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A)
- Dispense real-time PCR reaction solution (10 min): prepare a master mix of your cDNA sample and TB Green Premix Ex Taq II (Tli RNase Plus) (Cat. # RR820A) and dispense into a 96-well plate
- Add PrimerArray primers (5 min): use 4 µl per reaction
- Real-time PCR (1–2 hours): compatible with all commonly used real-time PCR systems
- Data analysis (30 min): PrimerArray analysis tool makes data analysis easy
PrimerArray Analysis Tool for Embryonic Stem Cells
The PrimerArray Analysis Tool for Embryonic Stem Cells facilitates data analysis, and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)
Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.
Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)
Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data from KhES-3 cells obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.
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Procedural overview for stem cell gene expression analysis by real-time RT-PCR
The overall workflow for the PrimerArray sets is straightforward:
- RNA extraction (30 min): we recommend NucleoSpin RNA II kits (Cat. # 740955.50) or RNAiso (Cat. # 9108, not available in all geographic locations)
- Reverse transcription (20 min): we recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A)
- Dispense real-time PCR reaction solution (10 min): prepare a master mix of your cDNA sample and TB Green Premix Ex Taq II (Tli RNase Plus) (Cat. # RR820A) and dispense into a 96-well plate
- Add PrimerArray primers (5 min): use 4 µl per reaction
- Real-time PCR (1–2 hours): compatible with all commonly used real-time PCR systems
- Data analysis (30 min): PrimerArray analysis tool makes data analysis easy
PrimerArray Analysis Tool for Embryonic Stem Cells
The PrimerArray Analysis Tool for Embryonic Stem Cells facilitates data analysis, and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)
Pluripotency-related gene expression was compared in the human ES cell lines KhES-1 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.
Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016)
Pluripotency-related gene expression was compared in the human ES cell lines KhES-3 in different stages of differentiation using PrimerArray Embryonic Stem Cells (Cat. # PH016). RNA was prepared from cultured ES cells at an early passage (early), at a late passage (late), or after differentiation (diff). Expression data from KhES-3 cells obtained from microarray analysis and the PrimerArray data were highly correlated, as shown in this figure.
