pRI 201 DNA Vectors
pRI 201 DNA Vectors
Brand: Takara Bio.
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pRI 201 DNA Vectors
pRI 201 DNA transformation vectors
The Ri Plasmid pRI 201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI 101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5'-untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI101 vector series.
Multigene transformation with a single vector can be achieved by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the vector's second multiple cloning site (MCS2) located downstream of the HSP terminator.
We offer two types of the pRI 201 DNA vectors, pRI 201-AN DNA and pRI 201-ON DNA. pRI 201-AN DNA contains an Arabidopsis ADH-derived 5'-UTR (AtADH 5'-UTR) and is suitable for dicotyledonous plant transformation. pRI 201-ON DNA contains a rice ADH-derived 5'-UTR (OsADH 5'-UTR) and is suitable for monocotyledonous plant transformation. The pRI 201-AN DNA and pRI 201-ON DNA binary vectors for plant transformation have a mutant-type replication origin (Ri ori) from the Rhizobium rhizogenes Ri plasmid. These vectors also contain a replication origin (ColE1 ori) derived from the pUC plasmid; the ColE1 ori allows high copy number replication in E. coli. The vectors' multiple cloning sites, located near the right border (RB) of T-DNA, allows stable target gene integration into the plant chromosome.
Overview
- Use pRI 201-AN DNA to transform dicotyledonous plant species
- Use pRI 201-ON DNA to transform monocotyledonous plant species
- Integrate multiple transgenes using a single vector
- High copy number (ColE1 ori) replication in E. coli
Storage
These vectors should be stored at –20°C and used within 2 years of receipt.
Product citations
Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells. Plant Cell Physiol. 51, 328–32 (2010).
Nishiguchi, R., Takanami, M. & Oka, A. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b. MGG Mol. Gen. Genet. 206, 1–8 (1987).
Satoh, J., Kato, K. & Shinmyo, A. The 5'-untranslated region of the tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plant. J. Biosci. Bioeng. 98, 1–8 (2004).
Sugio, T. et al. Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells. J. Biosci. Bioeng. 109, 170–3 (2010).
Sugio, T., Satoh, J., Matsuura, H., Shinmyo, A. & Kato, K. The 5'-untranslated region of the Oryza sativa alcohol dehydrogenase gene functions as a translational enhancer in monocotyledonous plant cells. J. Biosci. Bioeng. 105, 300–2 (2008).
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