pRI 101 DNA transformation vectors

pRI 101 DNA vectors are binary plant transformation vectors intended for foreign gene expression in plant cells. These agrobacterium-mediated plant transformation vectors carry the 35S promoter of cauliflower mosaic virus (CaMV) and the 5' non-coding region (5'-UTR) of the alcohol dehydrogenase (ADH) gene. The 5'-UTR of ADH functions as a translation enhancer in plants. We offer two types of pRI 101 DNA vectors—the pRI 101-AN DNA vector for dicotyledonous plants, such as tobacco or Arabidopsis, and the pRI 101-ON DNA vector for monocotyledonous plants, such as rice. The pRI 101-AN DNA vector carries the 5'-UTR of Arabidopsis ADH (AtADH 5'-UTR), and the pRI 101-ON DNA vector carries the 5'-UTR of rice ADH (OsADH 5'-UTR).

Although the pRI 101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium), they are high copy number plasmids because they contain the same replication origin as pUC-type plasmids (ColE1 ori). These vectors are also stably maintained in Rhizobium (Agrobacterium) containing the mutant-type replication origin Ri (Ri-ori). The pRI 101 DNA vectors are capable of stably integrating target genes into plant chromosomes because the vector cloning sites are located closer to the Right Border (RB) of T-DNA than the selection marker (NPT II) of the plant, so the target gene is not deleted.

Overview

• Agrobacterium-mediated plant transformation
• Easy and stable integration of dicot and monocot target genes into plant chromosomes
• Increased target gene expression via the 5'-UTR translation enhancer region
• Vector versatility for dicotyledonous and monocotyledonous plant cells