pRetroX-IRES-DsredExpress Vector
pRetroX-IRES-DsredExpress Vector
Brand: Takara Bio.
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pRetroX-IRES-DsredExpress Vector
The pRetroX-IRES-DsRedExpress vector is a bicistronic retroviral expression vector containing DsRed-Express as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (ECMV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and DsRed-Express to be independently translated from the same mRNA transcript. DsRed-Express is a human codon-optimized variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation.
Overview
- Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
- High titers when combined with the Retro-X Universal Packaging System
- Also, see our lentiviral and adenoviral options
Applications
- Reporter studies
- Monitoring subcellular localization
- Monitoring transduction efficiency and gene expression levels
Mechanism of IRES bicistronic expression from Retro-X retroviral vectors
Mechanism of IRES bicistronic expression from Retro-X retroviral vectors. The internal ribosome entry site (IRES) permits a protein of interest and a fluorescent protein to be independently translated from the same mRNA. SD = Splice Donor. SA = Splice Acceptor. ψ = packaging signal. LTR = long terminal repeat.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
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Mechanism of IRES bicistronic expression from Retro-X retroviral vectors
Mechanism of IRES bicistronic expression from Retro-X retroviral vectors. The internal ribosome entry site (IRES) permits a protein of interest and a fluorescent protein to be independently translated from the same mRNA. SD = Splice Donor. SA = Splice Acceptor. ψ = packaging signal. LTR = long terminal repeat.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
