pRetroQ-mCherry-N1 Vector
pRetroQ-mCherry-N1 Vector
Brand: Takara Bio.
In stock
SKU
pRetroQ-mCherry-N1 Vector
The pRetroQ-mCherry-N1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).
Overview
- Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
- High titers when combined with the Retro-X Universal Packaging System
- Also, see our lentiviral and adenoviral options
Applications
- Reporter studies
- Monitoring subcellular localization
- Monitoring transduction efficiency and gene expression levels
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
Write Your Own Review
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
