pRetroQ-DsRed Monomer-N1 Vector
pRetroQ-DsRed Monomer-N1 Vector
Brand: Takara Bio.
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pRetroQ-DsRed Monomer-N1 Vector
The pRetroQ-DsRed Monomer-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the N-terminus of the DsRed-Monomer protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.
Overview
- Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
- High titers when combined with the Retro-X Universal Packaging System
- Also, see our lentiviral and adenoviral options
Applications
- Reporter studies
- Monitoring subcellular localization
- Monitoring transduction efficiency and gene expression levels
DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
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DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
