pRetroQ-DsRed Monomer-C1 Vector
pRetroQ-DsRed Monomer-C1 Vector
Brand: Takara Bio.
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pRetroQ-DsRed Monomer-C1 Vector
The pRetroQ-DsRed Monomer-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the C-terminus of the DsRed-monomer protein. Together, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.
Overview
- Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
- High titers when combined with the Retro-X Universal Packaging System
- Also, see our lentiviral and adenoviral options
Applications
- Reporter studies
- Monitoring subcellular localization
- Monitoring transduction efficiency and gene expression levels
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
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Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
