pRetroQ-AcGFP1-C1 Vector
pRetroQ-AcGFP1-C1 Vector
Brand: Takara Bio.
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pRetroQ-AcGFP1-C1 Vector
The pRetroQ-AcGFP1-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the C-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.
Overview
- Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
- High titers when combined with the Retro-X Universal Packaging System
- Also, see our lentiviral and adenoviral options
Applications
- Reporter studies
- Monitoring subcellular localization
- Monitoring transduction efficiency and gene expression levels
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
Retroviral vectors available from Clontech
Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
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Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions
Retroviral vectors expressing AcGFP1 and DsRed-Monomer fluorescent protein fusions. pRetroQ-DsRed-Monomer-Golgi and pRetroQ-AcGFP1-Tubulin constructs were transfected individually into GP2-293 cells, and the VSV-G pseudotyped virus was harvested 48 hr posttransfection. These viral stocks were then used to coinfect HeLa cells, and expression was visualized 48 hr postinfection by fluorescent microscopy.
Retroviral vectors available from Clontech
Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately.
Retro-X retroviral vector maps
Retro-X retroviral vector maps. IRES-containing bicistronic retroviral vectors allow you to express two target genes (Vector A: pQCXIX) or a target gene and an antibiotic resistance gene (Vector B: pQCXIH,pQCXIN and pQCXIP). Fluorescent-fusion retroviral vectors express your protein of interest fused to a fluorescent protein (Vector C: pRetroQ-AcGFP1 N1/C1 and pRetroQ-DsRed Monomer N1/C1). Knockout RNAi-Ready pSIREN-RetroQ retroviral vectors are prelinearized and ready to accept a dsDNA oligonucleotide encoding a shRNA of your choice, (Vectors D and E: RNAi-Ready pSIREN-RetroQ-DsRedExpress or pSIREN-RetroQ-ZsGreen and RNAi-Ready pSIREN-RetroQ). To express shRNA under tight, inducible control, use the knockout Tet series of retroviral vectors (Vector F: Knockout RNAi-Ready pSIREN-RetroQ-TetH and pSIREN-RetroQ TetP).
