Prelude PreAmp Master Mix

Brand	Takara Bio

Prelude PreAmp Master Mix — sensitive, unbiased preamplification

Prelude PreAmp Master Mix uses an optimized 2X PCR mix for unbiased preamplification of over 100 targets, starting from a limited input of cDNA or gDNA (10 pg–100 ng). Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target enrichment analysis.

Overview

Least-biased preamplification—obtain at least 2–3X fewer outliers than other commercially available preamplification mixes
Greatest sensitivity—start with as little as 10 pg of cDNA or gDNA
Universal compatibility—preamplified samples can be used for real-time PCR on over 100 green intercalating dye or probe-based targets and for SNP genotyping and target enrichment
Convenient, ready-to-use master mix—just add cDNA or gDNA samples and primers, and then cycle 10–14 times

The benefits of preamplification

Figure 1. The benefits of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Data points for nonpreamplified genes with Ct >32 are not shown.

Uniform representation following preamplification

Figure 2. Uniform representation following preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

Uniformity following either 10 or 14 cycles of preamplification

Figure 3. Uniformity following either 10 or 14 cycles of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

Additional information

Prelude PreAmp Master Mix — sensitive, unbiased preamplification

Overview

The benefits of preamplification

Uniform representation following preamplification

Uniformity following either 10 or 14 cycles of preamplification

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