pQCXIX Retroviral Vector
pQCXIX Retroviral Vector
Brand: Takara Bio.
In stock
SKU
pQCXIX Retroviral Vector
The pQCXIX Retroviral Vector is a self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. It expresses two target genes from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A deletion in the 3' LTR provides the self-inactivating feature after reverse transcription of the expression cassette. This vector is useful for applications in which high titers and high-level bicistronic gene expression are desired.
Overview
- Retroviral vectors designed for high retroviral titers and high expression of your transgene
- Compatible with all Takara Bio brand retroviral packaging cell lines
- Vector information is listed on our vector information pages
- Fluorescent protein, MSCV, and tetracycline-inducible retroviral vectors are described elsewhere
Composite retroviral vector map of Retro-X Q Vector Set Set
Composite retroviral vector map of Retro-X Q Vector Set Set In this generalized version of the Q Vector Map, the common elements of all the vectors are represented. Immediately downstream of the CMV immediate early promoter, the multiple cloning site (MCS) is followed by a eukaryotic IRES that ensures a second ORF (an antibiotic resistance marker or another gene in the case of pQCXIX) is cotranscribed with the gene cloned into the MCS. The expression cassette has all of the essential elements for retroviral integration and expression.
Self-inactivation mechanism of the Retro-X Q retroviral vectors
Self-inactivation mechanism of the Retro-X Q retroviral vectors. The plus strand retroviral RNA (blue) is reverse transcribed. During integration, a circular intermediate is formed that results in duplication of the deletion in the U3 region of the 3' LTR. This inactivates the CMV/MSV hybrid promoter in the 5' LTR of the retrovirus so that transcription can only be driven from the internal promoter, PCMV IE.
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Composite retroviral vector map of Retro-X Q Vector Set Set
Composite retroviral vector map of Retro-X Q Vector Set Set In this generalized version of the Q Vector Map, the common elements of all the vectors are represented. Immediately downstream of the CMV immediate early promoter, the multiple cloning site (MCS) is followed by a eukaryotic IRES that ensures a second ORF (an antibiotic resistance marker or another gene in the case of pQCXIX) is cotranscribed with the gene cloned into the MCS. The expression cassette has all of the essential elements for retroviral integration and expression.
Self-inactivation mechanism of the Retro-X Q retroviral vectors
Self-inactivation mechanism of the Retro-X Q retroviral vectors. The plus strand retroviral RNA (blue) is reverse transcribed. During integration, a circular intermediate is formed that results in duplication of the deletion in the U3 region of the 3' LTR. This inactivates the CMV/MSV hybrid promoter in the 5' LTR of the retrovirus so that transcription can only be driven from the internal promoter, PCMV IE.
