The pQCXIN Retroviral Vector is a self-inactivating retroviral Q expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. It expresses a target gene along with the gene for the neomycin resistance marker from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A deletion in the 3' LTR provides the self-inactivating feature after reverse transcription of the expression cassette. This vector is useful for applications in which high titers and high-level gene expression are desired.
Overview
Retroviral vectors designed for high retroviral titers and high expression of your transgene
Compatible with all Takara Bio brand retroviral packaging cell lines
Vector information is listed on our vector information pages
Composite retroviral vector map of Retro-X Q Vector Set Set
Composite retroviral vector map of Retro-X Q Vector Set Set In this generalized version of the Q Vector Map, the common elements of all the vectors are represented. Immediately downstream of the CMV immediate early promoter, the multiple cloning site (MCS) is followed by a eukaryotic IRES that ensures a second ORF (an antibiotic resistance marker or another gene in the case of pQCXIX) is cotranscribed with the gene cloned into the MCS. The expression cassette has all of the essential elements for retroviral integration and expression.
The pQCXIN Retroviral Vector is a self-inactivating retroviral Q expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. It expresses a target gene along with the gene for the neomycin resistance marker from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A deletion in the 3' LTR provides the self-inactivating feature after reverse transcription of the expression cassette. This vector is useful for applications in which high titers and high-level gene expression are desired.
Overview
Retroviral vectors designed for high retroviral titers and high expression of your transgene
Compatible with all Takara Bio brand retroviral packaging cell lines
Vector information is listed on our vector information pages
Composite retroviral vector map of Retro-X Q Vector Set Set
Composite retroviral vector map of Retro-X Q Vector Set Set In this generalized version of the Q Vector Map, the common elements of all the vectors are represented. Immediately downstream of the CMV immediate early promoter, the multiple cloning site (MCS) is followed by a eukaryotic IRES that ensures a second ORF (an antibiotic resistance marker or another gene in the case of pQCXIX) is cotranscribed with the gene cloned into the MCS. The expression cassette has all of the essential elements for retroviral integration and expression.