pPAmCherry-N1 Vector
pPAmCherry-N1 Vector
Brand: Takara Bio.
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pPAmCherry-N1 Vector
pPAmCherry-N1 Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.
The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
