pPAmCherry-Mem Vector
pPAmCherry-Mem Vector
Brand: Takara Bio.
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pPAmCherry-Mem Vector
pPAmCherry-Mem Vector is mammalian expression vector encoding a fusion protein of PAmCherry and the N-terminal 20 amino acids of neuromodulin (GAP-43). PAmCherry is a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. The GAP-43 fragment contains a signal for posttranslational palmitoylation of cysteins 3 and 4 that targets the fusion protein to the plasma membrane.
The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
