Poly(A) Polymerase
Poly(A) Polymerase
Brand: Takara Bio.
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Poly(A) Polymerase
Poly(A) Polymerase
Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3' termini of RNA, effectively adding a poly(A) tail to RNA. The enzyme uses single-stranded RNA as a primer during poly(A) tailing. Poly(A) Polymerase is supplied in a buffer of 25 mM Tris-HCl (pH 7.9), 500 mM NaCl, 1 mM EDTA, 0.1 mM DTT, and 50% glycerol.
Applications
- Adding a poly(A) tail to RNA
- 3'-end RNA labeling
Product citations
Gething, M. J., Bye, J., Skehel, J. & Waterfield, M. Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus. Nature 287, 301–6 (1980).
Krug, M. S. & Berger, S. L. First-strand cDNA synthesis primed with oligo(dT). Methods Enzymol. 152, 316–25 (1987).
Winter, G. & Brownlee, G. G. 3'End labelling of RNA with 32P suitable for rapid gel sequencing. Nucleic Acids Res. 5, 3129–39 (1978).
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