pNY326 can replicate in B. choshinensis cells and its size (3.4 kb) is much smaller than that of pNCMO2 (5.2 kb). In addition, the promoter activity of the vector is substantially weaker than that of pNCMO2. It can be used to express toxic proteins. pNY326 exhibits stable productivity and is especially suited to large scale culture. The expression plasmid must be constructed in one step using B. choshinensis as the host. Because the transformation efficiency must be high, the use of Brevibacillus Competent Cells (105 transformants/μg DNA; Cat. #HB116) is recommended.
pNY326 can replicate in B. choshinensis cells and its size (3.4 kb) is much smaller than that of pNCMO2 (5.2 kb). In addition, the promoter activity of the vector is substantially weaker than that of pNCMO2. It can be used to express toxic proteins. pNY326 exhibits stable productivity and is especially suited to large scale culture. The expression plasmid must be constructed in one step using B. choshinensis as the host. Because the transformation efficiency must be high, the use of Brevibacillus Competent Cells (105 transformants/μg DNA; Cat. #HB116) is recommended.
