pNI is a shuttle vector between B. choshinensis and E. coli, as well as an intracellular expression vector. Expression plasmids can be constructed by using E. coli as the host, then transferred into B. choshinensis cells. pNI contains the P2 promoter, which does not work in E. coli but works as a strong promoter in B. choshinensis cells, enabling efficient protein production.
pNI is a shuttle vector between B. choshinensis and E. coli, as well as an intracellular expression vector. Expression plasmids can be constructed by using E. coli as the host, then transferred into B. choshinensis cells. pNI contains the P2 promoter, which does not work in E. coli but works as a strong promoter in B. choshinensis cells, enabling efficient protein production.