pmR-ZsGreen1 Vector
pmR-ZsGreen1 Vector
Brand: Takara Bio.
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pmR-ZsGreen1 Vector
pmR-ZsGreen1 is a mammalian expression vector designed to constitutively coexpress a microRNA (miRNA) of interest and the ZsGreen1 fluorescent protein. ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. The unmodified vector will express ZsGreen1 in mammalian cells.
Overview
- Strong coexpression of miRNA and a bright fluorescent protein
- Clone your miRNA into the 3' UTR of mCherry or ZsGreen1
- Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy
Applications
- Over-expression of microRNAs to study gene or microRNA function
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript. miRNA expression can be selected for and/or verified in transfected cells by monitoring red or green fluorescence.
The pmR-mCherry and pmR-ZsGreen1 Vectors
The pmR-mCherry and pmR-ZsGreen1 Vectors. Map of the vectors (Panel A). Cells transfected with the vectors express your miRNA and exhibit red or green fluorescence (Panel B).
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The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript. miRNA expression can be selected for and/or verified in transfected cells by monitoring red or green fluorescence.
The pmR-mCherry and pmR-ZsGreen1 Vectors
The pmR-mCherry and pmR-ZsGreen1 Vectors. Map of the vectors (Panel A). Cells transfected with the vectors express your miRNA and exhibit red or green fluorescence (Panel B).
