pmR-mCherry Vector
pmR-mCherry Vector
Brand: Takara Bio.
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pmR-mCherry Vector
pmR-mCherry is a mammalian expression vector designed to constitutively coexpress a microRNA (miRNA) of interest and the mCherry fluorescent protein. mCherry is a mutant fluorescent protein derived from the bright red fluorescent protein, DsRed. The unmodified vector can be used to express mCherry in mammalian cells.
Overview
- Strong coexpression of miRNA and a bright fluorescent protein
- Clone your miRNA into the 3' UTR of mCherry or ZsGreen1
- Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy
Applications
- Over-expression of microRNAs to study gene or microRNA function
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript. miRNA expression can be selected for and/or verified in transfected cells by monitoring red or green fluorescence.
The pmR-mCherry and pmR-ZsGreen1 Vectors
The pmR-mCherry and pmR-ZsGreen1 Vectors. Map of the vectors (Panel A). Cells transfected with the vectors express your miRNA and exhibit red or green fluorescence (Panel B).
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The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript
The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript. miRNA expression can be selected for and/or verified in transfected cells by monitoring red or green fluorescence.
The pmR-mCherry and pmR-ZsGreen1 Vectors
The pmR-mCherry and pmR-ZsGreen1 Vectors. Map of the vectors (Panel A). Cells transfected with the vectors express your miRNA and exhibit red or green fluorescence (Panel B).
