pmOrange2 Vector
pmOrange2 Vector
Brand: Takara Bio.
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pmOrange2 Vector
pmOrange2 is a Living Colors fluorescent protein vector which encodes mOrange2, a variant of mOrange which has been modified for improved stability. mOrange2 is derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mOrange2 coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmOrange2 is primarily intended to serve as a source of mOrange2 cDNA.
Overview
- Bright orange fluorescent protein monomers ideal for reporter studies
- mOrange2 excitation and emission maxima are 549 and 565 nm, respectively
- Can be detected on a Western blot using our mCherry antibody
Applications
- Fusions
- Tracking cell, organelle, and protein movement
- Bright reporter for mammalian cells
Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877–82 (2002).
Shaner, N. C. et al. Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat. Methods 5, 545–551 (2008).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
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Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
