pmOrange2-C1 Vector
pmOrange2-C1 Vector
Brand: Takara Bio.
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pmOrange2-C1 Vector
pmOrange2-C1 is a Living Colors fluorescent protein mammalian expression vector designed to express a protein of interest fused to the C-terminus of mOrange2, a variant of mOrange which has been modified for improved stability. mOrange2 is derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. The unmodified vector can be used to express mOrange2 in mammalian cells.
Overview
- Bright orange fluorescent protein monomers ideal for reporter studies
- mOrange2 excitation and emission maxima are 549 and 565 nm, respectively
- Can be detected on a Western blot using our mCherry antibody
Applications
- Fusions
- Tracking cell, organelle, and protein movement
- Bright reporter for mammalian cells
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877–82 (2002).
Shaner, N. C. et al. Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat. Methods 5, 545–551 (2008).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
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Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
