pmBanana Vector
pmBanana encodes mBanana, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mBanana coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmBanana is primarily intended to serve as a source of mBanana cDNA.
Overview
- Bright yellow fluorescent protein
- mBanana excitation and emission maxima are 540 and 553 nm, respectively
Applications
- Fusions
- Protein localization
- General reporter for mammalian cells
- Monitoring transfection efficiencies
Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnol. 22(12):1567–1572 (2004).
Wang, L. et al. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Nat. Acad. Sci. USA. 101(48):16745–16749 (2004).
Shu, X. et al. Novel Chromophores and Buried Charges Control Color in mFruits. Biochemistry 45(32):9639–9647 (2006).
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Nat. Acad. Sci. USA. 99(12):7877–7882 (2002).
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Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
