TALON PMAC Magnetic Phospho Enrichment Kit
TALON PMAC Magnetic Phospho Enrichment Kit
Brand: Takara Bio.
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TALON PMAC Magnetic Phospho Enrichment Kit
TALON PMAC Magnetic Phospho Enrichment Kit
The kit provides a straightforward, metal affinity-based procedure for isolating microgram quantities of phosphorylated proteins from mammalian cells and tissues. It supplies a complete set of buffers along with phospho-specific magnetic beads for enrichment of all types of phosphoproteins, both cytosolic and membrane-bound, that contain a phosphorylated amino acid side chain-including serine, threonine, or tyrosine.
Overview
- A simple, metal affinity-based procedure for microscale phosphoprotein purification
- Quick and easy enrichment from any cell or tissue sample
- Nondenaturing protocol maintains protein conformation and solubility
- Eluted samples ideal for small volume applications
Applications
- Isolation and enrichment of all types of phosphoproteins.
Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis
Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis. The TALON PMAC Magnetic Phospho Enrichment Kit can be used with any mammalian cell type or tissue sample. The yield of phosphorylated protein varies with different cell lines.
Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit
Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit. Proteins extracted from HEK 293 cells in Extraction/Loading Buffer containing protease inhibitors and a phosphatase inhibitor (10 mM sodium fluoride), were mixed with 200 ?l of a 5% suspension of Phospho Magnetic Beads at room temperature for 30 min, followed by washing and elution. The extract (Lanes 1), flowthrough (Lanes 2), and eluates (Lanes 3) were then analyzed by Western blotting using antibodies specific for phosphorylated AKT (Ser 473) in Panel A, P-AKT (Thr 308) in Panel B, P-PTEN (Ser 380) in Panel C, and P-GSK3 beta (Ser 9) in Panel D; the band at 51 kDa is due to cross-reactivity of the antibody with P-GSK3a.
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Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis
Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis. The TALON PMAC Magnetic Phospho Enrichment Kit can be used with any mammalian cell type or tissue sample. The yield of phosphorylated protein varies with different cell lines.
Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit
Highly effective enrichment of phosphorylated proteins using the TALON PMAC Magnetic Phospho Enrichment Kit. Proteins extracted from HEK 293 cells in Extraction/Loading Buffer containing protease inhibitors and a phosphatase inhibitor (10 mM sodium fluoride), were mixed with 200 ?l of a 5% suspension of Phospho Magnetic Beads at room temperature for 30 min, followed by washing and elution. The extract (Lanes 1), flowthrough (Lanes 2), and eluates (Lanes 3) were then analyzed by Western blotting using antibodies specific for phosphorylated AKT (Ser 473) in Panel A, P-AKT (Thr 308) in Panel B, P-PTEN (Ser 380) in Panel C, and P-GSK3 beta (Ser 9) in Panel D; the band at 51 kDa is due to cross-reactivity of the antibody with P-GSK3a.
