This lentiviral expression vector encodes the ZsGreen1 fluorescent protein tag. The coding sequence for this very bright green fluorescent protein (2.5x brighter than EGFP) was modified to include human codon usage preferences, which allow improved expression in mammalian cells. Inserting a cDNA in the MCS upstream of the ZsGreen1 coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.
To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shotsand the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
This lentiviral expression vector encodes the ZsGreen1 fluorescent protein tag. The coding sequence for this very bright green fluorescent protein (2.5x brighter than EGFP) was modified to include human codon usage preferences, which allow improved expression in mammalian cells. Inserting a cDNA in the MCS upstream of the ZsGreen1 coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.
To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shotsand the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.