pLVX-IRES-tdTomato Vector
pLVX-IRES-tdTomato Vector
Brand: Takara Bio.
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pLVX-IRES-tdTomato Vector
The pLVX-IRES-tdTomato Vector is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing dividing or nondividing mammalian cells. The vector contains an internal ribosomal entry site (IRES) which allows a gene-of-interest and the tdTomato fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. When used with the Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line (Cat. No. 632180), the vector generates high titers of replication-incompetent, VSV-G-pseudotyped lentivirus.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
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tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
