pLVX-DsRed-Monomer-N1 Vector
pLVX-DsRed-Monomer-N1 Vector
Brand: Takara Bio.
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pLVX-DsRed-Monomer-N1 Vector
The pLVX-DsRed-Monomer-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pLVX-DsRed-Monomer-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence. The cDNA is subsequently expressed as a fusion to the N-terminus of the DsRed-Monomer protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.
Lentiviral vector delivery of fluorescently labeled protein expression to human neural progenitor cells
Lentiviral vector delivery of fluorescently labeled protein expression to human neural progenitor cells. Neurospheres were allowed to differentiate on laminin-coated plates and then coinfected with equivalent amounts of Lenti-X viruses produced from a pLVX-AcGFP1-Actin vector and a pLVX-DsRed-Monomer-Nuc vector. AcGFP1-labeled actin allows clear visualization of the cytoskeleton (green), while DsRed-Monomer-labeled nuclei (red) are also visible.
Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
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Lentiviral vector delivery of fluorescently labeled protein expression to human neural progenitor cells
Lentiviral vector delivery of fluorescently labeled protein expression to human neural progenitor cells. Neurospheres were allowed to differentiate on laminin-coated plates and then coinfected with equivalent amounts of Lenti-X viruses produced from a pLVX-AcGFP1-Actin vector and a pLVX-DsRed-Monomer-Nuc vector. AcGFP1-labeled actin allows clear visualization of the cytoskeleton (green), while DsRed-Monomer-labeled nuclei (red) are also visible.
Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
