Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface. iMatrix-511 is a recombinant fragment of laminin-511 which acts as a substrate for stem cell culture media for ESCs and iPSCs. iMatrix-511 is chemically defined, animal-free, and xeno-free.
Overview
Chemically defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture
Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin
Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging
Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers
Applications
Stem cell culture under chemically defined, animal-free, xeno-free conditions
Ido, H. et al. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin ?? chains in integrin binding by laminins. J. Biol. Chem.282, 11144-11154 (2007).
Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun.3, 1236 (2012).
Taniguchi, Y. et al. The C-terminal region of laminin ?? chains modulates the integrin binding affinities of laminins. J. Biol. Chem.284, 7820-7831 (2009).
Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface. iMatrix-511 is a recombinant fragment of laminin-511 which acts as a substrate for stem cell culture media for ESCs and iPSCs. iMatrix-511 is chemically defined, animal-free, and xeno-free.
Overview
Chemically defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture
Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin
Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging
Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers
Applications
Stem cell culture under chemically defined, animal-free, xeno-free conditions
Ido, H. et al. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin ?? chains in integrin binding by laminins. J. Biol. Chem.282, 11144-11154 (2007).
Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun.3, 1236 (2012).
Taniguchi, Y. et al. The C-terminal region of laminin ?? chains modulates the integrin binding affinities of laminins. J. Biol. Chem.284, 7820-7831 (2009).
