PicoPLEX Single Cell WGA Kit

The PicoPLEX Single Cell WGA Kit uses a single-tube protocol developed specifically for reproducible amplification of single-copy genomic DNA (gDNA) starting with 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification, which results in libraries with very low background and typical yields of 8–12 µg of product in under 3 hours. This system is best suited for applications in which high yield is most important.

Overview

• Reproducible results from single cells (or 6–50 pg of DNA)
• Superior reproducibility of allele representation
• Easy to use and automate
• Unambiguous results at all resolutions (e.g., microarray and PCR)

Applications

• CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
• Target enrichment or amplicon enrichment for mutation detection
• SNP genotyping

Suitable Inputs

• Mammalian cells (1–10 cells)
• Bacterial cells (1,000–10,000 cells)
• Isolated DNA (6–50 pg of human DNA)
• Sorted chromosomes
• Intact or fragmented, single- or double-stranded DNA