The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations.
Overview
Reproducible results from single cells (or 6–50 pg of DNA)
Superior reproducibility of allele representation
Easy to use and automate
Unambiguous results at all resolutions (e.g., microarray and PCR)
Applications
CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
Target enrichment or amplicon enrichment for mutation detection
SNP genotyping
Suitable Inputs
Mammalian cells (1–10 cells)
Bacterial cells (1,000–10,000 cells)
Isolated DNA (6–50 pg of human DNA)
Sorted chromosomes
Intact or fragmented, single- or double-stranded DNA
The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations.
Overview
Reproducible results from single cells (or 6–50 pg of DNA)
Superior reproducibility of allele representation
Easy to use and automate
Unambiguous results at all resolutions (e.g., microarray and PCR)
Applications
CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
Target enrichment or amplicon enrichment for mutation detection
SNP genotyping
Suitable Inputs
Mammalian cells (1–10 cells)
Bacterial cells (1,000–10,000 cells)
Isolated DNA (6–50 pg of human DNA)
Sorted chromosomes
Intact or fragmented, single- or double-stranded DNA