PicoPLEX Gold Single Cell DNA-seq Kit
PicoPLEX Gold Single Cell DNA-seq Kit
Brand: Takara Bio.
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PicoPLEX Gold Single Cell DNA-seq Kit
Designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms
PicoPLEX Gold Single Cell DNA-Seq Kit
The PicoPLEX Gold Single Cell DNA-Seq Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on innovative PicoPLEX technology and yields libraries with superior reproducibility from 1–5 mammalian cells or genome equivalents of gDNA as input, providing reliable results from challenging samples such as formalin-fixed single cells. Single index, dual index, and unique dual index kits are available and must be purchased separately.
The kit features a streamlined workflow consisting of four steps—from input to amplified NGS libraries—that can be completed in less than four hours. The kit uses high-fidelity enzymes and optimized primers and is suitable for accurate and reproducible profiling of copy number variants (CNVs), single-nucleotide variants (SNVs), indels, and other small structural variants. The kit is compatible with single cells and purified DNA from humans, animals, and bacteria, as well as single flow-sorted chromosomes.
Overview
- Highly reproducible and accurate CNV, SNV, and aneuploidy detection
- Outperforms the leading MDA technology for SNV and CNV detection
- Streamlined three-hour workflow with less than 30 minutes of hands-on time
- Highly uniform coverage with low allele drop-in and drop-out rate
Applications
Library preparation for the following cell types:
- Cancer research: circulating tumor cells, cells, or DNA from laser capture microdissection (LCM), and cells from fine needle aspirate (FNA)
- Reproductive health research: embryonic cells and circulating fetal cells
- Developmental biology: embryonic cells, stem cells, and rare cells
- Metagenomics and microbiology: microbial cells, microbiome and environmental samples
PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell
PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell. Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.
PicoPLEX Gold Single Cell DNA-Seq Kit workflow
PicoPLEX Gold Single Cell DNA-Seq Kit workflow. The four-step SMARTer PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.
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PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell
PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell. Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.
PicoPLEX Gold Single Cell DNA-Seq Kit workflow
PicoPLEX Gold Single Cell DNA-Seq Kit workflow. The four-step SMARTer PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.
