Phosphorylated linker
Phosphorylated linker
Brand: Takara Bio.
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Phosphorylated linker
pNotI phosphorylated linker
The pNotI phosphorylated linker is a cloning reagent containing a phosphate group at its 5'-end, which enables it to be used directly in linker ligation during cloning experiments. The in-frame cloning of a target gene into a vector can be easily performed by using a phosphorylated linker that results in the desired reading frame.
This lyophilized product is a single-stranded oligonucleotide. For use in downstream applications, dissolve in a buffer such as TE, incubate at 70°C for 10 minutes, and anneal by cooling slowly.
Linker sequence
Linker, pNotI (10 mer): pd(AGCGGCCGCT)
Purity
- Confirmed by HPLC
- Biological activity confirmed by self-ligation and restriction enzyme cleavage
Storage
–20°C
Product citations
Brown, N. L. & Smith, M. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I. FEBS Lett. 65, 284-7 (1976).
Roberts, R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13 Suppl, r165-200 (1985).
Smith, D. I., Blattner, F. R. & Davies, J. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 3, 343-53 (1976).
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