The pHEK293 Ultra Expression Vectors are plasmid vectors developed for transient overexpression of recombinant proteins in human HEK293 cells. Protein expression using these vectors is 2–10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters.
Exploits the HIV-1 TAR-Tat system to express a protein of interest at ultra-high levels; yields up to 10 times higher protein production than conventional CMV promoter-driven expression systems
Two options are available:
The pHEK293 Ultra Expression Vector I (Cat. # 3390) is a single plasmid for overexpression of a protein of interest in HEK293 cells
The pHEK293 Ultra Expression Vector II (Cat. # 3392) includes the pHEK293 Enhancer Vector; the expression level of the target protein can be optimized by varying the ratio of the two plasmids that are used for transfection
Applications
Ultra-high expression of recombinant proteins in HEK 293 cells
The pHEK293 Ultra Expression Vectors are plasmid vectors developed for transient overexpression of recombinant proteins in human HEK293 cells. Protein expression using these vectors is 2–10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters.
Exploits the HIV-1 TAR-Tat system to express a protein of interest at ultra-high levels; yields up to 10 times higher protein production than conventional CMV promoter-driven expression systems
Two options are available:
The pHEK293 Ultra Expression Vector I (Cat. # 3390) is a single plasmid for overexpression of a protein of interest in HEK293 cells
The pHEK293 Ultra Expression Vector II (Cat. # 3392) includes the pHEK293 Enhancer Vector; the expression level of the target protein can be optimized by varying the ratio of the two plasmids that are used for transfection
Applications
Ultra-high expression of recombinant proteins in HEK 293 cells