pHcRed1-DR is a promoterless vector that encodes HcRed1-DR, a destabilized variant of Heteractis crispa far-red fluorescent protein, HcRed1. HcRed1-DR contains a PEST degradation domain at its C-terminus, so it is rapidly turned over in mammalian cell culture. Because if its short half-life, HcRed1-DR is well suited for monitoring the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site, located upstream of the human codon-optimized HCRed1-DR gene. Without the addition of a functional promoter, this vector will not express HcRed1-DR.
Overview
Far-red fluorescent protein, ideal for in vivo imaging
HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
Vector map for destabilized fluorescent protein vectors pDsRed-Express-DR, pHcRed1-DR, and pZsGreen1-DR
Vector map for destabilized fluorescent protein vectors pDsRed-Express-DR, pHcRed1-DR, and pZsGreen1-DR. These fluorescent protein variants display rapid turnover rates.
pHcRed1-DR is a promoterless vector that encodes HcRed1-DR, a destabilized variant of Heteractis crispa far-red fluorescent protein, HcRed1. HcRed1-DR contains a PEST degradation domain at its C-terminus, so it is rapidly turned over in mammalian cell culture. Because if its short half-life, HcRed1-DR is well suited for monitoring the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site, located upstream of the human codon-optimized HCRed1-DR gene. Without the addition of a functional promoter, this vector will not express HcRed1-DR.
Overview
Far-red fluorescent protein, ideal for in vivo imaging
HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
Vector map for destabilized fluorescent protein vectors pDsRed-Express-DR, pHcRed1-DR, and pZsGreen1-DR
Vector map for destabilized fluorescent protein vectors pDsRed-Express-DR, pHcRed1-DR, and pZsGreen1-DR. These fluorescent protein variants display rapid turnover rates.