pGFPuv encodes a variant of Aequorea victoria green fluorescent protein (GFP) that has been optimized for brighter fluorescence when excited by standard UV light. The GFPuv coding sequence is flanked by separate MCSes at the 5' and 3' ends, so the GFPuv gene can be excised from pGFPuv. Alternatively, the GFPuv coding sequences can be amplified by PCR.

pGFPuv carries the "cycle 3" variant of GFP. This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43. The GFPuv gene can be easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusion protein is expressed from the lac promoter in E. coli. Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e., non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.

Overview